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KMID : 1041219880300010025
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Journal of Sericultural and Entomological Science
1988 Volume.30 No. 1 p.25 ~ p.32
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Study on the Juvenile Hormone Binding Protein in the Hemolymph of the Silkworm Larva, Bombyx mori.
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Abstract
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In order to examine a physiological role of juvenile(JH) binding proteins in the hemelymph of the silkworm larva, Bombyx mori, [3H] JH I incubated hemolymph was separated by polyacrylamide gel electrophoresis in the fifth-instar larva and the activity of the binding protein was analyzed using charcoal binding assay.
The results obtained were as follows;
1. The JH was bound by two protein fractions in the hemolymph of the fifth-instar larva; One was JH binding lipoprotein(JH-LP), thr other was JH specific binding protein (JHBP). Their relative mobility values (Rm) were 0.3~0.33 and 0.81~0.84, respectively. There were no valid differences in those values from developmental stages of both male and female silltworms.
2. Total protein contents of the hemolymph were gradually increased during the fifth-instar larva, while at the prepupa decreasd. The maximum ones were observed at the spinning period and the contents from female were much higher than those from the male.
3. JH binding activity per ml of the hemolymph was low in the early stage of the fifth-instar larva and its activity was maximized at the spinning period and at the prepupa slightly decreased.
4. There was a similar pattern between changes of the JH binding activity per ml of the homolymph and of the total protein contents of the hemolymph.
5. The JH binding activity per mg of the hemolymph proteins was high in the early stage of the fifth-instar larva, while from the 6th day of the fifth-instar larva to the prepupa its activity showed the lowest levels.
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